Whole Genome Sequencing (WGS)

Whole genome sequencing (WGS) determines the complete DNA sequence of an organism's genome at single-base resolution, providing a comprehensive view of all genetic information in a single experiment. This approach enables the detection of single nucleotide variants, insertions and deletions, structural variants, and copy number changes across the entire genome. It is a fundamental technology for disease research, precision medicine, evolutionary biology, and genomic surveillance, delivering the comprehensive genetic insights needed to understand biological function and genetic diversity.

Procaryote Organism

Rp. 6.300.000 ($ 350) per sample

40 days

Small-Size Genime Eucaryote Organism (<150Mbp)

Rp. 6.500.000 ($ 365) per sample

40 days

Large-Size Genime Eucaryote Organism (>150Mbp)

Start from: Rp. 37.000.000 ($ 2070) per sample

Start from: 40 days

Sample requirement: ≥40 ng/µL DNA in 20 µL volume (≥800 ng total), A260/280 = 1.8–2.0, A260/230 >2.0, fragment size >20 kb

Sample requirement: ≥50 ng/µL DNA in 20 µL volume (≥2 µg total DNA), A260/280 = 1.8–2.0, A260/230 >2.0, fragment size >20 kb

Sample requirement: 30-40 µg DNA in 20 µL, A260/280 = 1.8–2.0, A260/230 >2.0, DNA fragment N50 >50 kb (preferred >100 kb)

Method & platform: direct sequencing ONT

Method & platform: direct sequencing ONT

Method & platform: ultra long direct sequencing ONT

Default Deliverables

  • Assembled Genome
    FASTA format containing high-quality contigs, scaffolds, or chromosome-level assemblies.

  • Assembly Metrics Report
    Comprehensive quality assessment including N50/L50, total genome size, GC content, and genome completeness (BUSCO).

  • Annotation Files

    • GFF3/GTF: gene coordinates and structural features

    • CDS sequences (FNA): nucleotide coding regions

    • Protein sequences (FAA): translated gene products

  • Functional Annotation
    Functional characterisation of predicted genes, including Gene Ontology (GO), KEGG pathways, Pfam domains, InterPro annotations, and more.

  • Standard Variant Discovery
    Identification of genomic variations, including Average Nucleotide Identity (ANI), aligned portion, single nucleotide polymorphisms (SNPs), insertions and deletions (indels), and structural variants (SVs).

  • Phylogenetic Analysis
    Evolutionary relationship analysis based on conserved genes or whole-genome data. Outputs are provided in Newick (.nwk) format and as publication-ready images (.png).

Add-ons

  • Basecalling

  • Hybrid Assembly

  • Chromosome-level Scaffolding

  • Repeat Annotation

  • Epigenomic Analysis

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