Transcriptomic Sequencing (cDNA)

Rp. 6.800.000 ($ 380) per sample

40 days

Sample Requirement: cDNA ≥40 ng/µL in 20 µL volume, A260/280 = 1.8–2.0, A260/230 >2.0, RIN ≥7 (preferred ≥8), minimal genomic DNA contamination

Method & platform: Direct Sequencing ONT

Transcriptomic Sequencing (cDNA)

  • Quality-Controlled Sequence Files

    FASTA/FASTQ files containing filtered, trimmed, and quality-controlled cDNA sequencing reads generated from raw direct sequencing data before downstream expression analysis.

  • Aligned Transcript Read Files

    BAM/CRAM files containing cDNA sequencing reads aligned to the selected reference genome or transcriptome, including indexed alignment files for transcript visualisation and downstream analysis.

  • Transcript Assembly & Quantification Files

    Transcript assembly and abundance estimation files containing reconstructed transcripts, transcript isoforms, and quantified gene/transcript expression levels generated from cDNA sequencing data.

  • Differential Gene Expression Report

    Comprehensive analysis of significantly upregulated and downregulated genes across experimental conditions, including statistical significance, fold-change values, and expression profiling results.

  • Functional Enrichment Analysis

    Biological interpretation of expressed and differentially expressed genes, including Gene Ontology (GO), KEGG pathway enrichment, and functional category analysis.

Default Deliverables

Transcriptomic sequencing profiles the complete set of RNA transcripts within a sample by sequencing complementary DNA (cDNA), providing a comprehensive view of gene expression, transcript diversity, and regulatory activity. It is a powerful approach for functional genomics, biomarker discovery, disease research, and understanding cellular responses.

Metatranscriptomic Sequencing (cDNA)

Metatranscriptomic (cDNA)

Rp. 6.800.000 ($ 380) per sample

40 days

Sample Requirement: cDNA ≥40 ng/µL in 20 µL volume, A260/280 = 1.8–2.0, A260/230 >2.0, RIN ≥7 (preferred ≥8), minimal short-fragment contamination

Method & platform: Amplicon Sequencing ONT

  • Quality-Controlled Sequence Files

    FASTA/FASTQ files containing filtered, trimmed, and quality-controlled metatranscriptomic sequencing reads generated from raw environmental or host-associated cDNA sequencing data before downstream functional analysis.

  • Functional Gene Expression Analysis

    Quantitative analysis of actively expressed microbial genes and metabolic functions, including pathway activity, enzyme classification, and community-level functional profiling.

  • Differential Functional Activity Report

    Comparative analysis of microbial transcriptional activity across samples or conditions, including differentially expressed functional pathways, genes, and metabolic responses.

Default Deliverables

Metatranscriptomic sequencing profiles the complete set of actively expressed RNA transcripts (cDNA) within microbial communities, revealing real-time functional activity and gene expression without the need for cultivation. It is a powerful approach for microbiome research, host–microbe interaction studies, environmental monitoring, and functional ecosystem analysis.

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