Metagenomics Sequencing

Rp. 6.800.000 ($ 380) per sample

40 days

Sample Requirement: Total Extract DNA ≥40 ng/µL in 20 µL volume, A260/280 = 1.8–2.0, A260/230 >2.0, fragment size >10 kb

Method & platform: Direct Sequencing ONT

Metagenomics Sequencing

  • Assembled Genome
    FASTA format containing high-quality contigs, scaffolds, or chromosome-level assemblies.

  • Assembly Metrics Report
    Comprehensive quality assessment including N50/L50, total genome size, GC content, and genome completeness (BUSCO).

  • Annotation Files

    • GFF3/GTF: gene coordinates and structural features

    • CDS sequences (FNA): nucleotide coding regions

    • Protein sequences (FAA): translated gene products

  • Functional Annotation
    Functional characterisation of predicted genes, including Gene Ontology (GO), KEGG pathways, Pfam domains, InterPro annotations, and more.

  • Standard Variant Discovery
    Identification of genomic variations, including Average Nucleotide Identity (ANI), aligned portion, single nucleotide polymorphisms (SNPs), insertions and deletions (indels), and structural variants (SVs).

  • Phylogenetic Analysis
    Evolutionary relationship analysis based on conserved genes or whole-genome data. Outputs are provided in Newick (.nwk) format and as publication-ready images (.png).

Default Deliverables

Metagenomic sequencing profiles the complete genetic content of microbial communities by sequencing all DNA present in an environmental or clinical sample, without the need for cultivation. This approach enables comprehensive taxonomic identification, functional gene characterization, genome reconstruction through metagenome-assembled genomes (MAGs), and the discovery of novel microorganisms. It is particularly powerful for microbiome research, environmental monitoring, pathogen surveillance, and uncovering the ecological and functional diversity of complex microbial ecosystems.

Amplicon Sequencing

Amplicon Sequencing

Rp. 2.000.000 ($ 115) per sample

40 days

Sample Requirement: Amplicon ≥25 ng/µL in 20 µL volume, A260/280 = 1.8–2.0, A260/230 >2.0

Method & platform: Amplicon Sequencing ONT

  • Quality-Controlled Sequence Files

    FASTA/FASTQ files containing filtered, trimmed, and quality-controlled amplicon sequencing reads generated from raw sequencing data.

  • OTU/ASV Feature Table

    Abundance tables representing microbial composition across all samples.

  • Taxonomic Classification

    Taxonomic assignment of microbial communities based on reference databases, including relative abundance estimation across multiple taxonomic levels.

  • Diversity Analysis

    Comprehensive microbial community analysis based on amplicon sequencing data, including taxonomic composition, relative abundance estimation, diversity assessment, and phylogenetic analysis.

  • Functional Prediction Analysis

    Predictive functional profiling of microbial communities inferred from marker gene data.

Default Deliverables

Amplicon sequencing characterizes microbial community composition by targeting and sequencing specific genetic marker regions, such as 16S rRNA, ITS, or 18S rRNA genes. This targeted approach provides a cost-effective and high-throughput means of identifying and comparing microbial taxa across diverse samples, making it ideal for biodiversity assessment, microbiome profiling, ecological studies, and monitoring changes in microbial community structure across environments or experimental conditions.

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